Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome

Quality Control in Mu DNA Transposition

Transposons are segments of DNA encoding protein the resulting Strand Transfer Complex (STC, also called factors that move the DNA segment to new locations in Type 2 complex) , the 59 ends of Mu DNA are still joined the genome. They are ubiquitous among organisms and to old flanking DNA sequence, while the 39 ends of Mu play an important role in genome evolution, including DNA are joined to new...

Mu in vitro DNA Transposition Applications in Protein Engineering

Visualizing Mu transposition: assembling the puzzle pieces.

Mobile genetic elements, once a curiosity of maize genetics, are now known to be remarkably prevalent in the genomes of organisms ranging from pathogenic bacteria to humans. The varied consequences of these elements include the alteration of gene expression and the spread of drug resistance genes among bacteria. Additionally, many viruses and bacteriophages that covalently integrate their own g...

3D reconstruction of the Mu transposase and the Type 1 transpososome: a structural framework for Mu DNA transposition.

Mu DNA transposition proceeds through a series of higher-order nucleoprotein complexes called transpososomes. The structural core of the transpososome is a tetramer of the transposase, Mu A, bound to the two transposon ends. High-resolution structural analysis of the intact transposase and the transpososome has not been successful to date. Here we report the structure of Mu A at 16-angstroms an...

Transposition of bacteriophage Mu in the Legionnaires disease bacterium.

Legionnaires disease is an acute respiratory disease that is often fatal for immunocompromised patients. The causative agent of this disease, Legionella pneumophila, is a Gram-negative bacterium that is present in a variety of aquatic environments. L. pneumophila is a facultative intracellular parasite; it grows within human phagocytic cells and eventually causes their destruction. In contrast ...